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Kevin Lerner's avatar

I sincerely appreciate the openness. Providing the methods, data, interpretations, and next steps is a great way to lean into scientific communication! Thanks for the post

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Nick Colaianni's avatar

This is a very nice write-up! Thanks for sharing your work. I have a few questions:

1) When cloning the DNA+barcode into the E.Coli strains, is it always placed in the same location?

2) You show good correlation between replicates across your time course, I was wondering if any genes showed positive correlation across time points. Particularly your high fitness effect barcodes at day 5.

3) You averaged your fitness per position, but I wonder if you analyzed data after collating positions/inserts by operons you would have more consistent results. Particularly when using two very different microbes where you may miss interesting things if the full operon isn't included in the DNA insert (thus making any one part non-functional)

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